BioTech Purification Method
By combining our uniquely cultured ERP KB-12 strain with the SepaTech Microbubble Technology, we have successfully enhanced both microbial retention and degradation efficiency beyond conventional methods.
Purification Process
- Initial Injection of ERP KB-12:
The ERP KB-12 strain (an aerobic microorganism) is specially cultured and directly injected into the contaminated site.
This initial injection ensures that the microorganisms reach the pollution source and begin the purification process promptly. - Secondary Injection with SepaTech Microbubbles:
Using the SepaTech Microbubble System, microorganisms are re-injected together with fine microbubbles.
Leveraging the buoyancy and dispersion properties of the bubbles, this method enables the even distribution of microorganisms throughout soil pores and groundwater pathways.
This two-step injection process allows microorganisms to settle not only at the contamination source but also across the surrounding environment. At the same time, oxygen delivered via microbubbles creates optimal conditions for microbial activity.
As a result, both oxygen supply and microbial dispersion are achieved simultaneously — promoting high degradation activity and purification performance.

Key Improvements Over Conventional Techniques
Traditional bioaugmentation often failed due to:
- Poor microbial delivery to target zones
- Difficulty of microbial retention in the environment
- Insufficient oxygen supply
Our method overcomes these limitations by:
- Direct initial injection into the pollution source
- Broad infiltration of microorganisms using microbubbles
- Continuous oxygen supply
This results in faster, broader, and more efficient purification.
Verified Results
In a field test conducted at a former factory site in Chuo Ward, Kobe City, the application of ERP KB-12 bioaugmentation led to a dramatic reduction in VOC-derived odors — from an odor index of approximately 960 to nearly zero within 24 hours.
Furthermore, monitoring confirmed that the low odor level persisted after treatment, indicating a lasting effect with no recurrence of odor.
You can review the detailed experimental results in this report.
NEXT:Practical Applications and Composting